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Glutathione does not just drop in total amount

After TMB substrate solution is added, only those wells that contain Human BMG/b2-MG, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color

After centrifugation at 500g for 15 min (27, 70), semen samples from each group ( n = 3) were collected with the number of frozen-thawed spermatozoa adjusted to a final concentration of 1 10 6 sperms/ml, added to the above fertilization droplet containing MII oocytes (repeated in triplicate, 205 oocytes for the NC group, 210 oocytes for the GSH group, 220 oocytes for the SeNPs group and 217 oocytes for the GSH + SeNPs group, repeated in triplicate) and cultured in a CO 2 incubator (38.5C, 5%CO 2 and 5%O 2 ) for 18 h

Bot Acta 105:174179 Koffler BE, Bloem E, Zellnig G, Zechmann B (2013) High resolution imaging of subcellular glutathione concentrations by quantitative immunoelectron microscopy in different leaf areas of Arabidopsis